Sites of synthesis of murine RNA tumor virus (oncornavirus) group-specific antigens.

SUMMARY The technique of autoradiognaphy of immunoelectro phonetic patterns was used to study sites of synthesis of mammary tumor virus group-specific antigen (MTV-sl) and munine leukemia virus group-specific antigen (MuL V. gsl) protein in C3H and AKR mice ofvanious ages. Synthesis of MTV-sI protein was seen only in the mam mary gland of tumor-free late pregnant and lactating C3H mice. In addition, mammary tumors were strongly positive for production of MTV-sl antigen. Production of MuL V-gs protein was found in spleen, thymus, lymph node, placenta, mammary gland, testis, and ovary of nonleukemic AKR, as well as in some testis and ovary cultures of C3H mice. Leukemic ARK lymph oid tissue showed much stronger labeling of MuL V-gs than normal. In general, tissues that tended to have high extract titers for MuL V-gs protein as tested by immuno fluorescent adsorption also appeared positive with respect